Objective To study the risk of systemic diffusion of mycobacterium tuberculosis after emergency operation for spinal tuberculosis with spinal cord injury.Methods 44 cases of spinal tuberculosis with spinal cord lesion who underwent emergency operation were selected as research group,and 59 cases of spinal tuberculosis with spinal cord injury who underwent selective operation were selected as control group.Mycobacterium tuberculosis DNA(MTB-DNA) was extracted from peripheral blood on one day before operation,the first day after operation and the seventh day after operation,respectively.Real-time fluorescence quantitative detection of MTB-DNA was carried out by PCR amplification.And the risk of systemic diffusion of mycobacterium tuberculosis after emergency surgery for spinal tuberculosis with spinal cord injury was evaluated by comparative analysis.Results The DNA positive rate of mycobacterium tuberculosis in peripheral blood was 50.0%(22/44),56.8%(25/44) and 31.8%(14/44) in the research group on one day before operation,the first day after operation and the seventh day after operation,respectively,and 49.1%(29/59),52.5%(31/59) and 33.9%(20/59) in the control group,respectively.There was no statistically significant difference in the contents of mycobacterium tuberculosis in peripheral blood in the research group on one day before operation,the first day after operation and the seventh day after operation(F=0.08,P=0.920).There was no statistically significant difference in the contents of mycobacterium tuberculosis in peripheral blood in the control group on one day before operation,the first day after operation and the seventh day after operation(F=1.29,P=0.084).In addition,there was no statistically significant difference in the contents of mycobacterium tuberculosis between the research group and the control group on one day before operation,the first day after operation and the seventh day after operation(t=1.415,0.158,0.144,P=0.078,0.874,0.889,respectively).Conclusion There was no drug resistance of mycobacterium tuberculosis in patients with spinal tuberculosis with spinal cord injury before operation,and regular anti-tuberculosis treatment was performed after operation.Difference in the contents of mycobacterium tuberculosis in peripheral blood before and after emergency operation and selective operation was not significant,which shows that emergency operation in patients with spinal tuberculosis with spinal cord injury does not cause the risk of systemic diffusion of mycobacterium tuberculosis.
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